Conjugation of 5(6)-carboxyfluorescein and 5(6)-carboxynaphthfluorescein with bovine serum albumin and their immobilization for optical pH sensing

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Publikace nespadá pod Filozofickou fakultu, ale pod Přírodovědeckou fakultu. Oficiální stránka publikace je na webu muni.cz.
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BIDMANOVÁ Šárka HLAVÁČEK Antonín DAMBORSKÝ Jiří PROKOP Zbyněk

Rok publikování 2012
Druh Článek v odborném periodiku
Časopis / Zdroj SENSORS AND ACTUATORS B: CHEMICAL
Fakulta / Pracoviště MU

Přírodovědecká fakulta

Citace
Doi http://dx.doi.org/10.1016/j.snb.2011.09.046
Obor Genetika a molekulární biologie
Klíčová slova pH indicator Fluorescence Optical sensor Immobilization Conjugation Fluorescein
Popis An approach for the immobilization of a pH indicator in optical pH sensors and biosensors was developed. Fluorescent dyes 5(6)-carboxyfluorescein and 5(6)-carboxynaphthofluorescein were conjugated with bovine serum albumin as a non-enzymatic scaffold protein and the conjugation procedure was optimized. Fluorescent properties, sensitivity to temperature and photostability of conjugates have been studied and characterized into details. The conjugates were immobilized on glass support by: (i) glutaraldehyde crosslinking or (ii) entrapment in sol–gel matrix ORMOCER with subsequent glutaraldehyde cross-linking. The response to pH and leaching potential were evaluated. The sensor layer, based on immobilized conjugates of 5(6)-carboxyfluorescein and bovine serum albumin, displayed rapid response over the pH range 4.0–9.0, making it compatible with a range of applications such as bioprocessing, clinical diagnostics or environmental monitoring. Immobilized conjugates of 5(6)-carboxynaphthofluorescein and bovine serum albumin showed an enhanced sensitivity in alkaline pH levels, response for glutaraldehyde crosslinking ranged from pH 9.1 to 11.0 and from 8.3 to 10.0 for the ORMOCER entrapment, however, control of the indicator leaching was essential.
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