Genome-wide transcriptional analysis of Drosophila larvae infected by entomopathogenic nematodes shows involvement of complement, recognition and extracellular matrix proteins

Logo poskytovatele

Varování

Publikace nespadá pod Filozofickou fakultu, ale pod Přírodovědeckou fakultu. Oficiální stránka publikace je na webu muni.cz.
Autoři

AREFIN Badrul KUČEROVÁ Lucie DOBEŠ Pavel MARKUS Robert STRNAD Hynek WANG Zhi HYRŠL Pavel ŽUROVEC Michal THEOPOLD Ulrich

Rok publikování 2014
Druh Článek v odborném periodiku
Časopis / Zdroj Journal of Innate Immunity
Fakulta / Pracoviště MU

Přírodovědecká fakulta

Citace
www http://www.karger.com/Article/FullText/353734
Doi http://dx.doi.org/10.1159/000353734
Obor Imunologie
Klíčová slova Basement membrane; Coagulation; Hemocyte; Insect immunity; Nematodes; Recognition molecule; Thioester-containing proteins
Popis Heterorhabditis bacteriophora is an entomopathogenic nematode (EPN) which infects its host by accessing the hemolymph where it releases endosymbiotic bacteria of the species Photorhabdus luminescens. We performed a genome-wide transcriptional analysis of the Drosophila response to EPN infection at the time point at which the nematodes reached the hemolymph either via the cuticle or the gut and the bacteria had started to multiply. Many of the most strongly induced genes have been implicated in immune responses in other infection models. Mapping of the complete set of differentially regulated genes showed the hallmarks of a wound response, but also identified a large fraction of EPN-specific transcripts. Several genes identified by transcriptome profiling or their homologues play protective roles during nematode infections. Genes that positively contribute to controlling nematobacterial infections encode: a homolog of thioester-containing complement protein 3, a basement membrane component (glutactin), a recognition protein (GNBP-like 3) and possibly several small peptides. Of note is that several of these genes have not previously been implicated in immune responses.
Související projekty:

Používáte starou verzi internetového prohlížeče. Doporučujeme aktualizovat Váš prohlížeč na nejnovější verzi.