Alkaloid chelirubine and DNA: Blue and red luminescence
| Název česky | Alkaloid chelirubin a DNA: Modrá a červená luminiscence |
|---|---|
| Autoři | |
| Rok publikování | 2013 |
| Druh | Článek v odborném periodiku |
| Časopis / Zdroj | Talanta |
| Fakulta / Pracoviště MU | |
| Citace | |
| www | http://www.sciencedirect.com/science/article/pii/S0039914012008764 |
| Doi | http://dx.doi.org/10.1016/j.talanta.2012.10.045 |
| Obor | Analytická chemie, separace |
| Klíčová slova | Alkaloid; Benzo[c]phenanthridine; Chelirubine; DNA; Intercalation; Fluorescence |
| Popis | Extracts from Himalayan herb Dicranostigma lactucoides containing alkaloid chelirubine have been used for centuries in Chinese herbal medicine. We have found a new employment for the alkaloid: it can be used as a DNA fluorescent probe showing blue (free form) and red (intercalated to DNA) luminescence emission after irradiation by violet light. Besides quantification of DNA (LOD = 6 ng.ml-1) it can also be used as a supravital cell probe because chelirubine molecules can effectively enter to the living cell through the cell membrane. |
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