Organic Co-solvents Affect Activity, Stability and Enantioselectivity of Haloalkane Dehalogenases
| Autoři | |
|---|---|
| Rok publikování | 2013 |
| Druh | Článek v odborném periodiku |
| Časopis / Zdroj | Biotechnology Journal |
| Fakulta / Pracoviště MU | |
| Citace | |
| Doi | http://dx.doi.org/10.1002/biot.201200378 |
| Obor | Biochemie |
| Klíčová slova | Haloalkane Dehalogenases |
| Popis | Haloalkane dehalogenases are microbial enzymes with a wide range of biotechnological applications. The use of organic co-solvents to solubilize their hydrophobic substrates is often necessary. In order to choose the most compatible co-solvent, the effects of fourteen co-solvents on activity, stability and enantioselectivity of three model enzymes, DbjA, DhaA and LinB, were evaluated. All co-solvents caused at high concentration loss of activity and conformational changes. The highest inactivation was induced by tetrahydrofuran, while more hydrophilic co-solvents, such as ethylene glycol and dimethyl sulfoxide, were more tolerated. The effects of co-solvents at low concentration were different for each enzyme-solvent pair. The increase in DbjA activity was induced by the majority of organic co-solvents tested, while activities of DhaA and LinB decreased at comparable concentrations of the same co-solvent. Moreover, high increase of DbjA enantioselectivity was observed. Ethylene glycol and 1,4-dioxane were shown to have the most positive impact on the enantioselectivity. The favourable influence of the co-solvents on both activity and enantioselectivity makes DbjA suitable for biocatalytic applications. This study represents the first investigation of the effects of organic co-solvents on the catalytic performance of haloalkane dehalogenases and will pave the way for their broader use in industrial processes. |
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