Electrochemical behaviour of 2,4-dinitrophenylhydrazi(o)ne asmulti-redox centre DNA label at mercury meniscus modified silversolid amalgam electrode

• Autoři: DAŇHEL Aleš; RAINDLOVA Veronika; HAVRAN Luděk; PIVOŇKOVÁ Hana; HOCEK Michal; FOJTA Miroslav
• Rok publikování: 2014
• Druh: Článek v odborném periodiku
• Časopis / Zdroj: Electrochimica Acta
• Fakulta / Pracoviště MU: Středoevropský technologický institut
• www: http://dx.doi.org/10.1016/j.electacta.2013.09.147
• Doi: http://dx.doi.org/10.1016/j.electacta.2013.09.147
• Obor: Elektrochemie
• Klíčová slova: DNA; Redox labeling; 2_4-Dinitrophenylhydrazine; Silver solid amalgam electrode; Voltammetry
• Popis: A detailed voltammetric study of 2,4-dinitrophenylhydrazine (DNPH), a DNPH modified nucleotide(dCDNPHMP) and a short single stranded DNA (ssDNA-DNPH) at mercury meniscus modified silver solid amalgam electrode (m-AgSAE) is presented in this work. Electrochemical behaviour of these compounds was studied using cyclic voltammetry (CV), adsorptive stripping CV and open circuit adsorptive transfer stripping CV. Successful incorpo-ration of the DNPH labeled nucleoside triphosphate into the DNA structure shows that selected DNApolymerase tolerates this bulky moiety and can be used, in combination with electrochemical methodsutilizing m-AgSAE, in further development of various applications.

Související projekty

• Centrum nových přístupů k bioanalýze a molekulární diagnostice