New sensitive detection method for lectin hemagglutination using microscopy.
| Název česky | Nová citlivá metoda pro lektinovou hemaglutinaci pomocí mikroskopu |
|---|---|
| Autoři | |
| Rok publikování | 2014 |
| Druh | Článek v odborném periodiku |
| Časopis / Zdroj | Microscopy research and technique |
| Fakulta / Pracoviště MU | |
| Citace | |
| www | http://dx.doi.org/10.1002/jemt.22407 |
| Doi | http://dx.doi.org/10.1002/jemt.22407 |
| Obor | Biochemie |
| Klíčová slova | hemagglutination; protein-carbohydrate interactions; blood group antigens; lectin |
| Popis | The blood group system AB0 is determined by the composition of terminal oligosaccharides on red blood cells. Thanks to this structural feature, these groups can be recognized by saccharide-recognizing compounds. Hemagglutination is a process in which blood cells are cross-linked via multivalent molecules, lectins e.g. A hemagglutination assay is commonly used for the detection of multivalent molecules that recognize blood cells, in order to search for their sugar specificity. It is traditionally performed on a microtiter plate, where the lectin solution is serially diluted and the lowest concentration of lectin causing agglutination is detected. We have developed a new way of detecting hemagglutination using microscopy, which was tested on purified lectins as well as cell lysates. Hemagglutination was performed on a microscope slide directly and detected using a microscope. Comparison with the standard hemagglutination assay using microtiter plates revealed that microscopic approach is faster and more robust and allows fast determination of lectin activities immediately in bacterial cytosols. |
| Související projekty: |