Acinetobacter bohemicus sp nov widespread in natural soil and water ecosystems in the Czech Republic

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Publikace nespadá pod Filozofickou fakultu, ale pod Středoevropský technologický institut. Oficiální stránka publikace je na webu muni.cz.
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KŘÍŽOVÁ Lenka MAIXNEROVÁ Martina ŠEDO Ondrej NEMEC Alexandr

Rok publikování 2014
Druh Článek v odborném periodiku
Časopis / Zdroj Systematic and Applied Microbiology
Fakulta / Pracoviště MU

Středoevropský technologický institut

Citace
www http://ac.els-cdn.com/S0723202014001015/1-s2.0-S0723202014001015-main.pdf?_tid=3acb56e0-c7dd-11e4-9ce5-00000aab0f01&acdnat=1426071551_9208211a79d54dbbf3b9f209899d12f7
Doi http://dx.doi.org/10.1016/j.syapm.2014.07.001
Obor Mikrobiologie, virologie
Klíčová slova rpoB; gyrB; 16S rRNA gene; ANI; Carbon source assimilation; MALDI-TOF MS
Popis We investigated the taxonomic status of a phenetically unique group of 25 Acinetobacter strains which were isolated from multiple soil and water samples collected in natural ecosystems in the Czech Republic. Based on the comparative sequence analyses of the rpoB, gyrB, and 16S rRNA genes, the strains formed a coherent and well separated branch within the genus Acinetobacter. The genomic uniqueness of the group at the species level was supported by the low average nucleotide identity values (<= 77.37%) between the whole genome sequences of strain ANC 3994(T) (NCBI accession no. APOH00000000) and the representatives of the known Acinetobacter species. Moreover, all 25 strains created a tight cluster clearly separated from all hitherto described species based on whole-cell protein profiling by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) and shared a unique combination of metabolic and physiological properties. The capacity to assimilate L-histidine and the inability to grow at 35 degrees C differentiated them from their phenotypically closest neighbor, Acinetobacter johnsonii. We conclude that the 25 strains represent a novel Acinetobacter species, for which the name Acinetobacter bohemicus sp. nov. is proposed. The type strain of A. bohemicus is ANC 3994(T) (=CIP 110496(T) = CCUG 63842(T) = CCM 8462(T)).
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