Telomere dynamics in the lower plant Physcomitrella patens

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Publikace nespadá pod Filozofickou fakultu, ale pod Středoevropský technologický institut. Oficiální stránka publikace je na webu muni.cz.
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FOJTOVÁ Miloslava SÝKOROVÁ Eva NAJDEKROVÁ Lucie POLANSKÁ Pavla ZACHOVÁ Dagmar VÁGNEROVÁ Radka ANGELIS Karel J. FAJKUS Jiří

Rok publikování 2015
Druh Článek v odborném periodiku
Časopis / Zdroj Plant Molecular Biology
Fakulta / Pracoviště MU

Středoevropský technologický institut

Citace
www http://download.springer.com/static/pdf/161/art%253A10.1007%252Fs11103-015-0299-9.pdf?originUrl=http%3A%2F%2Flink.springer.com%2Farticle%2F10.1007%2Fs11103-015-0299-9&token2=exp=1443784026~acl=%2Fstatic%2Fpdf%2F161%2Fart%25253A10.1007%25252Fs11103-015-02
Doi http://dx.doi.org/10.1007/s11103-015-0299-9
Obor Genetika a molekulární biologie
Klíčová slova Physcomitrella patens; Telomere maintenance; Telomerase; PpTERT structure; DSB repair mutants
Popis A comparative approach in biology is needed to assess the universality of rules governing this discipline. In plant telomere research, most of the key principles were established based on studies in only single model plant, Arabidopsis thaliana. These principles include the absence of telomere shortening during plant development and the corresponding activity of telomerase in dividing (meristem) plant cells. Here we examine these principles in Physcomitrella patens as a representative of lower plants. To follow telomerase expression, we first characterize the gene coding for the telomerase reverse transcriptase subunit PpTERT in P. patens, for which only incomplete prediction has been available so far. In protonema cultures of P. patens, growing by filament apical cell division, the proportion of apical (dividing) cells was quantified and telomere length, telomerase expression and activity were determined. Our results show telomere stability and demonstrate proportionality of telomerase activity and expression with the number of apical cells. In addition, we analyze telomere maintenance in mre11, rad50, nbs1, ku70 and lig4 mutants of P. patens and compare the impact of these mutations in double-strand-break (DSB) repair pathways with earlier observations in corresponding A. thaliana mutants. Telomere phenotypes are absent and DSB repair kinetics is not affected in P. patens mutants for DSB factors involved in non-homologous end joining (NHEJ). This is compliant with the overall dominance of homologous recombination over NHEJ pathways in the moss, contrary to the inverse situation in flowering plants.
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