Histone H1 Differentially Inhibits DNA Bending by Reduced and Oxidized HMGB1 Protein

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Publikace nespadá pod Filozofickou fakultu, ale pod Středoevropský technologický institut. Oficiální stránka publikace je na webu muni.cz.
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ŠTROS Michal POLANSKÁ Eva KUČÍREK Martin POSPÍŠILOVÁ Šárka

Rok publikování 2015
Druh Článek v odborném periodiku
Časopis / Zdroj Plos one
Fakulta / Pracoviště MU

Středoevropský technologický institut

Citace
www http://www.plosone.org/article/fetchObject.action?uri=info:doi/10.1371/journal.pone.0138774&representation=PDF
Doi http://dx.doi.org/10.1371/journal.pone.0138774
Obor Biochemie
Klíčová slova GROUP BOX DOMAINS; BINDING PROTEINS; LINKER HISTONES; CHROMATIN INTERACTIONS; LIVING CELLS; 2 MUTATIONS; A-DOMAIN; ASSOCIATION; HMG-1; LIGATION
Přiložené soubory
Popis HMGB1 protein and linker histone H1 have overlapping binding sites in the nucleosome. HMGB1 has been implicated in many DNA-dependent processes in chromatin involving binding of specific proteins, including transcription factors, to DNA sites pre-bent by HMGB1. HMGB1 can also act as an extracellular signaling molecule by promoting inflammation, tumor growth a metastasis. Many of the intra-and extracellular functions of HMGB1 depend on redox-sensitive cysteine residues of the protein. Here we report that mild oxidization of HMGB1 (and much less mutation of cysteines involved in disulphide bond formation) can severely compromise the functioning of the protein as a DNA chaperone by inhibiting its ability to unwind or bend DNA. Histone H1 (via the highly basic C-terminal domain) significantly inhibits DNA bending by the full-length HMGB1, and the inhibition is further enhanced upon oxidization of HMGB1. Interestingly, DNA bending by HMGB1 lacking the acidic C-tail (HMGB1 Delta C) is much less affected by histone H1, but oxidization rendered DNA bending by HMGB1 Delta C and HMGB1 equally prone for inhibition by histone H1. Possible consequences of histone H1-mediated inhibition of DNA bending by HMGB1 of different redox state for the functioning of chromatin are discussed.
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