Effects of the soil microbial community on mobile proportions and speciation of mercury (Hg) in contaminated soil

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Publikace nespadá pod Filozofickou fakultu, ale pod Přírodovědeckou fakultu. Oficiální stránka publikace je na webu muni.cz.
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SZÁKOVÁ Jiřina HAVLÍČKOVÁ Jitka ŠÍPKOVÁ Adéla GABRIEL Jiří ŠVEC Karel BALDRIAN Petr SYSALOVÁ Jiřina COUFALÍK Pavel ČERVENKA Rostislav ZVĚŘINA Ondřej KOMÁREK Josef TLUSTOŠ Pavel

Rok publikování 2016
Druh Článek v odborném periodiku
Časopis / Zdroj Journal of Environmental Science and Health - Part A Toxic/Hazardous Substances and Environmental Engineering
Fakulta / Pracoviště MU

Přírodovědecká fakulta

Citace
Doi http://dx.doi.org/10.1080/10934529.2015.1109413
Obor Analytická chemie, separace
Klíčová slova mercury; microbial community; mobility; rhizobox; speciation
Popis The precise characterization of the behavior of individual microorganisms in the presence of increased mercury contents in soil is necessary for better elucidation of the fate of mercury in the soil environment. In our investigation, resistant bacterial strains isolated from two mercury contaminated soils, represented by Paenibacillus alginolyticus, Burkholderia glathei, Burkholderia sp., and Pseudomonas sp., were used. Two differently contaminated soils (0.5 and 7mg kg(-1) total mercury) were chosen. Preliminary soil analysis showed the presence of methylmercury and phenylmercury with the higher soil mercury level. Modified rhizobox experiments were performed to assess the ability of mercury accumulating strains to deplete the mobile and mobilizable mercury portions in the soil by modification; microbial agar cultures were used rather than the plant root zone. A sequential extraction procedure was performed to release the following mercury fractions: water soluble, extracted in acidic conditions, bound to humic substances, elemental, and bound to complexes, HgS and residual. Inductively coupled plasma mass spectrometry (ICP-MS) and a single-purpose atomic absorption spectrometer (AMA-254) were applied for mercury determination in the samples and extracts. Gas chromatography coupled to atomic fluorescence spectrometry (GC-AFS) was used for the determination of organomercury compounds. The analysis of the microbial community at the end of the experiment showed a 42% abundance of Paenibacillus sp. followed by Acetivibrio sp., Brevibacillus sp., Cohnella sp., Lysinibacillus sp., and Clostridium sp. not exceeding 2% abundance. The results suggest importance of Paenibacillus sp. in Hg transformation processes. This genus should be tested for potential bioremediation use in further research.
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