The motility in selenidiid archigregarines (Selenidiidae), an early emerging group of Apicomplexa
| Autoři | |
|---|---|
| Rok publikování | 2016 |
| Druh | Konferenční abstrakty |
| Fakulta / Pracoviště MU | |
| Citace | |
| Popis | Apicomplexans belong to the most monitored group of protists, comprising exclusively parasites of human and animals. Gregarines represent one of the groups with a remarkable diversity a basal lineage restricted to invertebrate hosts. Archigregarines occur only in marine environments and are expected to be the most recent ancestor of gregarines and perhaps apicomplexans as a whole, because of their morphological and life history characteristics. In general, gregarines exhibit various forms of motility, which seem to depend on cytoskeletal structures (subpellicular microtubules, intermediate filaments, actin and myosin). Selenidiid archigregarines exhibit a pendular or rolling movement. Their surface, covered by a typical threemembrane pellicle displays broad folds, beneath each sets of parallel longitudinal subpellicular microtubules are present. In this study experiments using cytoskeletal drugs were performed on living individuals of Selenidium sp., parasitising the intestine of marine polychaete Pygospio elegans (Spionidae). Two probes, cytochalasine D and jasplakinolide, influencing the polymerisation of actin were applied to determine the role of actin filaments in motility. Furthermore, drugs that destroy existing microtubules and inhibit tubulin polymerization, oryzalin and colchicine, were used to confirm the role of subpellicular microtubules. Expected results of this study should verify the fundamental role of cytoskeletal proteins, actin and tubulin, in archigregarine motility and compare their movement mechanism to the substratedependent gliding machinery (= the so called “glideosome” concept) described in apicomplexan zoites. The authors acknowledge the financial support from a project No. GBP505/12/G112 (ECIP) from Czech Science Foundation, and Assoc. prof. T. G. Simdyanov for help with material collection. |
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