Monogenea: Parasites of fish and their unknown molecules
| Autoři | |
|---|---|
| Rok publikování | 2017 |
| Druh | Další prezentace na konferencích |
| Fakulta / Pracoviště MU | |
| Citace | |
| Popis | The majority of known monogenean species are ectoparasites of fish with monoxenous life cycle in freshwater or marine ecosystems. Some species are of high economic importance, they can be highly pathogenic to fish, which can result in losses in aquaculture. In contrast to these facts there is almost nothing known about the molecular nature of the interactions with fish hosts and functions of particular monogeneans bioactive molecules. In a first part of our pilot study of monogeneans molecules we adopted the NGS techniques in order to get the high quality “monogenean-polyopisthocotylean genome/transcriptome matrix”. In a second part of our study this platform was used for the identification of the monogeneans´ dominant protein molecules followed by their further molecular/biochemical characterization. We adopted experimental organism Eudiplozoon nipponicum (Platyhelminthes, Monogenea) in order to obtain high quality genome, transcriptome and proteome for further annotation, development of robust gene/protein muster platform for this group of organisms. Subsequently, we used this dataset in combination with bioinformatics approach enable in silico determination of antigenic proteins - vaccine candidates. In addition to the bioinformatics analyses we performed mass spectrometric identification of particular protein compounds in excretory-secretory products from E. nipponicum in order to confirm the presence of previously predicted antigenic molecules. Finally, some of the predicted molecules have been prepared in recombinant form and characterized (serpins, cystatins). |
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