Characterization of two highly divergent lineages of exfoliative toxin B-encoding plasmids revealed in impetigo strains of Staphylococcus aureus

Varování

Publikace nespadá pod Filozofickou fakultu, ale pod Přírodovědeckou fakultu. Oficiální stránka publikace je na webu muni.cz.
Autoři

BOTKA Tibor RŮŽIČKOVÁ Vladislava SVOBODOVÁ Karla PANTŮČEK Roman PETRÁŠ Petr ČEJKOVÁ Darina DOŠKAŘ Jiří

Rok publikování 2017
Druh Článek ve sborníku
Konference EMBO | EMBL Symposium: New Approaches and Concepts in Microbiology
Fakulta / Pracoviště MU

Přírodovědecká fakulta

Citace
www https://www.embo-embl-symposia.org/symposia/2017/EES17-05/index.html
Obor Genetika a molekulární biologie
Klíčová slova ETB plasmid; exfoliative toxin B; phylogeny; genome analysis
Popis Staphylococcal epidermolytic diseases are caused by S. aureus strains producing exfoliative toxins A and B encoded by mobile genetic elements: plasmids and prophages. We analyzed various etb gene-positive plasmids isolated from the impetigo strains implicated in outbreaks of pemphigus neonatorum in Czech maternity hospitals. Most of the plasmids were related to archetypal plasmid pETBTY4. They shared a same core sequence, but they differed from each other in the content of additional DNA regions such as antibiotic resistance-encoding transposons. The ETB plasmids of this lineage were carried by strains of clonal complex CC121. Some of these strains also harbored exfoliative toxin A-encoding prophages. A novel distinct type of ETB plasmid originated from a prophageless impetigo strain was isolated and characterized in detail. Carrying conjugative cluster genes, as well as new variants of ETB and EDIN genes, it could be regarded as a source of a new lineage of ETB plasmids. Based on our findings, we have designed a helpful detection assay, which facilitates the precise identification of the all described types of ETB plasmids.
Související projekty:

Používáte starou verzi internetového prohlížeče. Doporučujeme aktualizovat Váš prohlížeč na nejnovější verzi.