A preliminary study of endocannabinoid system regulation in psychosis: Distinct alterations of CNR1 promoter DNA methylation in patients with schizophrenia

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Publikace nespadá pod Filozofickou fakultu, ale pod Lékařskou fakultu. Oficiální stránka publikace je na webu muni.cz.
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ADDARIO D. MICALE Vincenzo DI BARTOLOMEO M. ŠTARK Tibor PUCCI M. ŠULCOVÁ Alexandra PALAZZO M. BABINSKÁ Zuzana CREMASCHI L. DRAGO F. ALTAMURA C.A. MACCARRONE M. DELL'OSSO B.

Rok publikování 2017
Druh Článek v odborném periodiku
Časopis / Zdroj Schizophrenia Research
Fakulta / Pracoviště MU

Lékařská fakulta

Citace
Doi http://dx.doi.org/10.1016/j.schres.2017.01.022
Obor Psychiatrie, sexuologie
Klíčová slova Endocannabinoid system (ECS); Cannabinoid receptor type-1; DNA methylation; Schizophrenia; Methylazoxymethanol (MAM) rat model
Popis Compelling evidence supports the involvement of the endocannabinoid system(ECS) in psychosis vulnerability. We here evaluated the transcriptional regulation of ECS components in human peripheral blood mononuclear cells (PBMCs) obtained from subjects suffering from bipolar disorder, major depressive disorder and schizophrenia, focusing in particular on the effects of DNA methylation. We observed selective alterations of DNA methylation at the promoter of CNR1, the gene coding for the type-1 cannabinoid receptor, in schizophrenic patients (N = 25) with no changes in any other disorder. We confirmed the regulation of CNR1 in a well-validated animal model of schizophrenia, induced by prenatal methylazoxymethanol (MAM) acetate exposure (N = 7 per group) where we found, in the prefrontal cortex, a significant increase in CNR1 expression and a consistent reduction in DNA methylation at specific CpG sites of gene promoter. Overall, our findings suggest a selective dysregulation of ECS in psychosis, and highlight the evaluation of CNR1 DNA methylation levels in PBMCs as a potential biomarker for schizophrenia. (C) 2017 Elsevier B.V. All rights reserved.
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