The study of perifosine in colorectal carcinoma spheroids by MALDI TOF MSI and confocal microscopy
| Název česky | Studie perifosinu v sferoidech kolorektálního karcinomu pomocí MALDI TOF zobrazovací hmotnostní spektrometrie a konfokální mikroskopie |
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| Autoři | |
| Rok publikování | 2018 |
| Druh | Konferenční abstrakty |
| Fakulta / Pracoviště MU | |
| Citace | |
| Popis | In our study, the spheroids derived from colorectal carcinoma and potential cancerostatic perifosine were studied. The cellular objects were treated by perifosine for selected time interval (2, 8 and 24 hours) and stained by two viability markers one hour before processing for matrix-assisted laser desorption ionization mass spectrometry imaging (MALDI MSI), according to previously published protocol. The analysis of sections under confocal microscope was carried out before matrix application (in our case DHB sublimation). The examination of selected fluorescent molecules provided information about cell viability across the section, which was then related to perifosine distribution detected by MALDI MSI. Interestingly, both perifosine and the live cell marker were found to diffuse from spheroid to gelatine during sample preparation. |
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