Conditioning sciatic nerve injury triggers regeneration program of the primary sensory neurons also in the dorsal root ganglia non-associated with damaged nerve
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| Rok publikování | 2018 |
| Druh | Konferenční abstrakty |
| Fakulta / Pracoviště MU | |
| Citace | |
| Popis | The activation of the intrinsic regenerative program of DRG neurons is at least partly associated with upregulation of IL-6. The IL-6 mRNA and protein were upregulated not only in DRG associated with injured sciatic nerve, but also in remote cervical DRG. The goal of present experiments was to investigate if a sciatic nerve injury can activate the neuronal regeneration program not only in lumbar but also remote cervical DRG. Activation of intrinsic regeneration capacities of cervical DRG neurons induced by a sciatic nerve injury (compression or transection for 7 days) was evidenced by upregulation of GAP-43, SCG-10 and STAT-3 mRNAs and proteins. Increased level of IL-6 protein in the cerebrospinal fluid following sciatic nerve injury and subsequent activation of STAT-3 as well as increased expression of GAP-43 and SCG-10 in DRG after intrathecal injection of IL-6 showed that this cytokine can penetrate DRG to activate neuronal regenerative program also in remote DRG neurons. These molecular changes were correlated with extent of regenerated axons after ulnar nerve crush following prior sciatic nerve injury. Moreover, increased axon regeneration capacity of cervical DRG neurons initiated by conditioning sciatic nerve injury was also confirmed by an in vitro test. Our results demonstrate that unilateral sciatic nerve injury induces pro-regenerative state also in remote DRG neurons probably by IL-6 released into the cerebrospinal fluid. |
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