Microbial Contamination of Ancient Human Dental Calculus Studied by Scanning Electron Microscopy and Metagenomic Approach

• Autoři: CHOCHOLOVÁ Eva; FIALOVÁ Dana; DROZDOVÁ Eva; SKOUPÝ Radim; BRZOBOHATÁ Kristýna; ZWINSOVÁ Barbora; VÍDEŇSKÁ Petra; CHOCHOLA Václav
• Rok publikování: 2019
• Druh: Konferenční abstrakty
• Fakulta / Pracoviště MU: Přírodovědecká fakulta
• Popis: Dental calculus has great potential in the research of oral microbiome in past populations. Alongside the advantages of novel molecular approaches applied on ancient human dental calculus, we encounter the drawbacks as well. One of the main issues is contamination. There is a possibility of detection of microbial contamination by scanning electron microscopy prior to subsequent expensive molecular analysis. Although decontamination is always part of the workflow, the metagenomic analysis points out the residual microbial contamination. This study presents the means of detecting contamination and precautions to be taken for the best prevention of it.

Související projekty

• Identifikace bakteriálně přenosných nemocí z historického zubního kamene