PHOTON-UPCONVERTING NANOPARTICLES AS A NOVEL BACKGROUND-FREE LABEL IN IMMUNOASSAYS

Varování

Publikace nespadá pod Filozofickou fakultu, ale pod Středoevropský technologický institut. Oficiální stránka publikace je na webu muni.cz.
Autoři

FARKA Zdeněk HLAVACEK A. MICKERT M.J. SKLÁDAL Petr GORRIS Hans-Heiner

Rok publikování 2018
Druh Článek ve sborníku
Konference 9TH INTERNATIONAL CONFERENCE ON NANOMATERIALS - RESEARCH & APPLICATION (NANOCON 2017)
Fakulta / Pracoviště MU

Středoevropský technologický institut

Citace
Klíčová slova Photon-upconversion nanoparticle; immunoassay; bioconjugation; antibody; diclofenac
Popis Photon-upconverting nanoparticles (UCNPs) have become an attractive label in immunoassays because their anti-Stokes luminescence can be excited by the NIR laser and detected in the VIS region without optical background interference. Further advantages of UCNPs include good photostability, large anti-Stokes shifts, and multiple narrow emission bands that can be used for multiplexed detection. We have developed a competitive upconversion-linked immunosorbent assay (ULISA) for detection of the pharmaceutical diclofenac (DCF) in surface waters. Silica-coated UCNPs (50 nm in diameter) with carboxyl groups on the surface were synthesized and conjugated with the secondary anti-IgG antibody. The structure and monodispersity of the nanoconjugates was studied by TEM and agarose gel electrophoresis. Using a highly affine anti-DCF primary antibody, the optimized ULISA provided a detection limit of 50 pg.mL(-1).
Související projekty:

Používáte starou verzi internetového prohlížeče. Doporučujeme aktualizovat Váš prohlížeč na nejnovější verzi.