Species-specific PCR assay for the detection of Babesia odocoilei

Varování

Publikace nespadá pod Filozofickou fakultu, ale pod Přírodovědeckou fakultu. Oficiální stránka publikace je na webu muni.cz.

Autoři	BURGESS Hilary J. LOCKERBIE Betty P. AYALEW Lisanework E. DIBERNARDO Antonia HRAZDILOVÁ Kristýna MODRÝ David BOLLINGER Trent K.
Rok publikování	2021
Druh	Článek v odborném periodiku
Časopis / Zdroj	Journal of Veterinary Diagnostic Investigation
Fakulta / Pracoviště MU	Přírodovědecká fakulta
Citace
www	https://doi.org/10.1186/s13071-017-2064-1
Doi	http://dx.doi.org/10.1177/10406387211032927
Klíčová slova	Babesia odocoilei; cervids; PCR; specificity
Popis	We developed a PCR assay for the detection of Babesia odocoilei based on the 18S rRNA gene. Multiple specimens of B. odocoilei were examined, and the assay consistently produced a small specific PCR product of 306 bp. The PCR assay was also challenged with DNA from 13 other Babesia species and 2 Theileria species, originating from 10 different host species; however, nonspecific DNA amplification and multiple banding patterns were observed, and the amplicon banding patterns varied between different isolates of the same species. Sensitivity was determined to be 6.4 pg of DNA, and an estimated 0.0001% parasitism. This assay can be utilized for species-specific differential detection of B. odocoilei.
Související projekty:	CEITEC 2020