Global Transcriptomic Analysis of Bacteriophage-Host Interactions between a Kayvirus Therapeutic Phage and Staphylococcus aureus

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Publikace nespadá pod Filozofickou fakultu, ale pod Přírodovědeckou fakultu. Oficiální stránka publikace je na webu muni.cz.
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FINSTRLOVÁ Adéla MAŠLAŇOVÁ Ivana BLASDEL REUTER Bob G. DOŠKAŘ Jiří GÖTZ Friedrich PANTŮČEK Roman

Rok publikování 2022
Druh Článek v odborném periodiku
Časopis / Zdroj Microbiology Spectrum
Fakulta / Pracoviště MU

Přírodovědecká fakulta

Citace
www https://journals.asm.org/doi/10.1128/spectrum.00123-22
Doi http://dx.doi.org/10.1128/spectrum.00123-22
Klíčová slova phage-host interactions; Staphylococcus phages; Kayvirus; RNA-Seq; viral transcription; noncoding RNA; prophages; bacteriophage therapy; Staphylococcus aureus; transcriptome
Přiložené soubory
Popis Kayviruses are polyvalent broad host range staphylococcal phages with a potential to combat staphylococcal infections. However, the implementation of rational phage therapy in medicine requires a thorough understanding of the interactions between bacteriophages and pathogens at omics level. To evaluate the effect of a phage used in therapy on its host bacterium, we performed differential transcriptomic analysis by RNA-Seq from bacteriophage K of genus Kayvirus infecting two Staphylococcus aureus strains, prophage-less strain SH1000 and quadruple lysogenic strain Newman. The temporal transcriptional profile of phage K was comparable in both strains except for a few loci encoding hypothetical proteins. Stranded sequencing revealed transcription of phage noncoding RNAs that may play a role in the regulation of phage and host gene expression. The transcriptional response of S. aureus to phage K infection resembles a general stress response with differential expression of genes involved in a DNA damage response. The host transcriptional changes involved upregulation of nucleotide, amino acid and energy synthesis and transporter genes and downregulation of host transcription factors. The interaction of phage K with variable genetic elements of the host showed slight upregulation of gene expression of prophage integrases and antirepressors. The virulence genes involved in adhesion and immune evasion were only marginally affected, making phage K suitable for therapy.
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