Stealthy Player in Lipid Experiments? EDTA Binding to Phosphatidylcholine Membranes Probed by Simulations and Monolayer Experiments

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Publikace nespadá pod Filozofickou fakultu, ale pod Středoevropský technologický institut. Oficiální stránka publikace je na webu muni.cz.
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VAZDAR K. TEMPRA C. OLŻYŃSKA A. BIRIUKOV Denys CWIKLIK L. VAZDAR M.

Rok publikování 2023
Druh Článek v odborném periodiku
Časopis / Zdroj Journal of Physical Chemistry B
Fakulta / Pracoviště MU

Středoevropský technologický institut

Citace
www https://pubs.acs.org/doi/10.1021/acs.jpcb.3c03207
Doi http://dx.doi.org/10.1021/acs.jpcb.3c03207
Klíčová slova phosphatidylcholine monolayers; EDTA; molecular simulation dynamics; Langmuir monolayer experiments
Přiložené soubory
Popis Ethylenediaminetetraacetic acid (EDTA) is frequently used in lipid experiments to remove redundant ions, such as Ca2+, from the sample solution. In this work, combining molecular dynamics (MD) simulations and Langmuir monolayer experiments, we show that on top of the expected Ca2+ depletion, EDTA anions themselves bind to phosphatidylcholine (PC) monolayers. This binding, originating from EDTA interaction with choline groups of PC lipids, leads to the adsorption of EDTA anions at the monolayer surface and concentration-dependent changes in surface pressure as measured by monolayer experiments and explained by MD simulations. This surprising observation emphasizes that lipid experiments carried out using EDTA-containing solutions, especially of high concentrations, must be interpreted very carefully due to potential interfering interactions of EDTA with lipids and other biomolecules involved in the experiment, e.g., cationic peptides, that may alter membrane-binding affinities of studied compounds.
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