Rapid determination of uracil in biological fluids at mercury thin film electrode for early detection of potential 5-fluorouracil toxicity due to dihydropyrimidine dehydrogenase deficiency

Varování

Publikace nespadá pod Filozofickou fakultu, ale pod Lékařskou fakultu. Oficiální stránka publikace je na webu muni.cz.
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ASHRAFI Amir M SELCUK Ozge MUKHERJEE Atripan UNAL Didem Nur KURBANOGLU Sevinc USLU Bengi JUŘICA Jan PEKARKOVA Jana RICHTERA Lukas ADAM Vojtech

Rok publikování 2024
Druh Článek v odborném periodiku
Časopis / Zdroj Biosensors and Bioelectronics
Fakulta / Pracoviště MU

Lékařská fakulta

Citace
www https://www.sciencedirect.com/science/article/pii/S0956566324005505
Doi http://dx.doi.org/10.1016/j.bios.2024.116545
Klíčová slova Mercury thin film electrode; Adsorptive stripping voltammetry; Uracil determination; Dihydropyrimidine dehydrogenase phenotyping; 5-Fluorouracil-based therapies
Popis Determination of plasma uracil was reported as a method for evaluation of Dihydropyrimidine dehydrogenase (DPD) activity that is highly demanded to ensure the safe administration of 5-fluorouracil (5-FU)-based therapies to cancer patients. This work reports the development of a simple electroanalytical method based on adsorptive stripping square wave voltammetry (AdSWV) at mercury film-coated glassy carbon electrode (MF/GCE) for the highly sensitive determination of uracil in biological fluids that can be used for diagnosis of decreased DPD activity. Due to the formation of the HgII–Uracil complex at the electrode surface, the accuracy of the measurement was not affected by the complicated matrices in biological fluids including human serum, plasma, and urine. The high sensitivity of the developed method results in a low limit of detection (?1.3 nM) in human plasma samples, falling below the practical cut-off level of 15 ng mL-1 (?0.14 µM). This threshold concentration is crucial for predicting 5-FU toxicity, as reported in buffer, and ?1.15% in biological samples), and accuracy (recovery percentage close to 100%).

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