Application of Micellar Electrokinetic Chromatography for the Analysis of Artichoke Extract.
| Autoři | |
|---|---|
| Rok publikování | 2000 |
| Druh | Článek ve sborníku |
| Konference | Biochemie a molekulární biologie na prahu nového tisíciletí |
| Fakulta / Pracoviště MU | |
| Citace | |
| Obor | Biochemie |
| Klíčová slova | artyčok; micelární elektrokinetická chromatografie |
| Popis | Artichoke (Cynara) is well known as a vegetable, but it has also application in pharmacology. The choleretic and hypocholesterolemic actions of the artichoke extract are already known. The present research is being carried out into the antioxidative and hepatoprotective effects of such extracts. These activities have been attributed to the phenolic compounds present in the extract and to its caffeoylquinic derivatives. Micellar electrokinetic capillary chromatography (MECC) has been developed as a promising method for the analysis of the Artichoke extract. Separation conditions have been optimised with respect to different parameters including pH and ion strength of the background electrolyte, SDS concentration and temperature. A buffer consisting of 100 mM SDS in 20 mM sodium dihydrogen phosphate, 20 mM disodium tetraborate pH 8.5 was found to be the most suitable electrolyte for this separation. An applied voltage of 28 kV (positive polarity) and a temperature of 25 oC gave the best separation of the caffeoylquinic acid derivatives. |
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