Immunomicroscopical observation on the nervous system of adult Eudiplozoon nippnicum (Monogenea: Diplozoidae)
| Autoři | |
|---|---|
| Rok publikování | 2001 |
| Druh | Článek v odborném periodiku |
| Časopis / Zdroj | International Journal for Parasitology |
| Fakulta / Pracoviště MU | |
| Citace | |
| Obor | Zoologie |
| Klíčová slova | monogenean neurobiology; cholinesterase; serotonin; neuropeptides; confocal microscopy; immunogold labeling |
| Popis | Neuronal pathways have been examined in adult Eudiplozoon nipponicum (Monogenea: Diplozoidae) using cytochemistry interfaced with confocal scanning laser microscopy, in an attempt to ascertain the status of the nervous system. Peptidergic and serotinergic innervation was demonstrated by indirect immunocytochemistry and cholinnergic componets by enzyme cytochemical methodology; post-embeding electron microscopical immunogold labelling revealed neuropeptide immunoreactivity at the subcellular level. All three classes of neuronal mediators were identified throught both central and peripheral elements of a well-differentiated orthogonal nervous systems.There was considerable overlap in the staining patterns for cholinergic and peptinergic compoments; while dual immunostaining revealed serotonin immunoreactivity to be largely confined to a separate set of neurons. The subcellular distribution of immunoreactivity to the flatworm neuropeptide, GYIRFamide, confirmed neuropeptide localisation in dense-cored vesicles in the majority of the axons and terminal varicosities of both central and peripheral nervous systems. Result revealed an extensive and chemically diverse nervous system and suggest that pairing of individuals involves fusion of central nerve elements; it is likely also that there is continuity between the peripheral nervous systems of the two partner worms. |
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