Determination of dimethylated arginines in human plasma by HPLC with fluorescent detection
| Název česky | Stanovení dimethylovaných argininů v lidské plazmě pomocí HPLC s fluorescenční detekcí |
|---|---|
| Autoři | |
| Rok publikování | 2007 |
| Druh | Článek ve sborníku |
| Konference | Book of Abstracts |
| Fakulta / Pracoviště MU | |
| Citace | |
| www | Advances in chromatography and electrophoresis 2007 & Chiranal 2007 |
| Obor | Analytická chemie, separace |
| Klíčová slova | assymetric dimethylarginine; HPLC |
| Popis | Asymmetric dimethylarginine (ADMA) is a posttranslationally modified form of arginine that is generated in all types of cells during the process of degradation methylated proteins. We report here development of a new HPLC method for the determination of ADMA using fluorescent detection. After solid-phase extraction procedure and evaporation of the eluate under gentle stream of nitrogen the reconstituted samples were derivatised with an ortho-phthaldialdehyde reagent containing 3-mercaptopropionic acid. Formed derivatives were analysed on a Hydro-RP column using isocratic elution and fluorescence detection. |
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