Characterization and chromosomal location of powdery mildew resistance genes from wild barley PI282605
| Název česky | Charakterizace a chromozomová lokalizace genů odolnosti k padlí travnímu donora ječmene PI282605 |
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| Autoři | |
| Rok publikování | 2009 |
| Druh | Článek v odborném periodiku |
| Časopis / Zdroj | Journal of Plant Diseases and Protection |
| Fakulta / Pracoviště MU | |
| Citace | |
| www | https://link.springer.com/article/10.1007/BF03356319 |
| Obor | Genetika a molekulární biologie |
| Klíčová slova | Blumeria graminis f.sp. hordei; DNA markers; genetic analysis; Hordeum vulgare; resistance genes |
| Popis | The objective of this work was to find the identity of resistance genes against powdery mildew (Blumeria graminis f. sp. hordei) by mapping in an F2 population (229 plants) derived from a cross between winter barley (Hordeum vulgare L.) variety Tiffany and the wild barley (H. vulgare ssp. spontaneum) accession PI282605. The present study was aimed at introduction of powdery mildew resistance genes from wild barley into cultivated barley and their prospective exploitation in breeding. This cross demonstrated a two-locus model of resistance after genetic analysis. Microsatellite markers and two newly developed cleaved amplified polymorphic sequence markers were used for mapping with 115 F2 plants. By interval mapping on the short arm of chromosome 1H, one resistance gene was found to be linked with GBM1007 and Bmac0213. A second gene was mapped between EBmag0794 and Bmag0206 on the short arm of chromosome 7H at a distance of 2 cM from Bmag0206. A third gene was assigned to a linkage with cMWG682 on the short arm of chromosome 2H. Discrepancies between two loci identified by genetic analysis and three loci by molecular analysis are discussed. The most effective gene participating in powdery mildew resistance was that on chromosome 2H (67% of phenotypic variation). |
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