Electrophysiological Effects of Voltage Sensitive Dye di-4-ANNEPS in Rabbit and Guinea Pig Isolated Heart

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Publikace nespadá pod Filozofickou fakultu, ale pod Lékařskou fakultu. Oficiální stránka publikace je na webu muni.cz.
Název česky Elektrofyziologické účinky napěťově citlivého barviva di-4-ANEPPS studované na modelu izolovaného srdce králíka a morčete
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FIALOVÁ Kateřina KOLÁŘOVÁ Jana SEKORA Jiří TANNENBERG Milan NOVÁKOVÁ Marie PROVAZNÍK Ivo

Rok publikování 2010
Druh Článek v odborném periodiku
Časopis / Zdroj Analysis of Biomedical Signals and Images
Fakulta / Pracoviště MU

Lékařská fakulta

Citace
Obor Fyziologie
Klíčová slova voltage sensitive dye; isolated heart
Popis Voltage-sensitive dye (VSD) di-4-ANEPPS is currently used in our laboratory for recording of monophasic action potentials (MAPs) in isolated hearts. In this study, its electrophysiological effects on rabbit and guinea pig myocardium were compared during staining with VSD and washout from it. Electrograms were recorded and heart rate changes and the types and frequency of arrhythmias were evaluated. Althought certain electrophysiological changes were present in the hearts of both species, these changes are mostly reversible, especially in rabbit hearts. Thus, rabbit myocardium can be considered a reliable model for electrophysiological studies, especially when generation and propagation of the impulse is studied. It may be concluded that rabbit isolated heart is more suitable model for electrophysiological studies in the isolated heart model than guinea pig heart.
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