Reciprocal and multi-species chromosome BAC painting in crucifers (Brassicaceae)
| Autoři | |
|---|---|
| Rok publikování | 2010 |
| Druh | Článek v odborném periodiku |
| Časopis / Zdroj | Cytogenetic and Genome Research |
| Fakulta / Pracoviště MU | |
| Citace | |
| Obor | Genetika a molekulární biologie |
| Klíčová slova | Arabidopsis halleri; Arabidopsis thaliana; BAC FISH; Brassicaceae; Collinearity; Comparative chromosome painting; Plant cytogenetics |
| Popis | The paper reports on principles of multi-species and multi-directional painting in crucifer species (Brassicaceae). We have successfully tested chromosome-specific BAC contigs of A. thaliana (n = 5) and A. halleri (n = 8) as probes for reciprocal BAC painting. Furthermore,BAC contigs of both Arabidopsis species were applied as multi-species painting probes to a third crucifer species, Noccaea caerulescens (n = 7), revealing their shared chromosome homeology. Specifically, we found homeology across portions of chromosomes At2, Ah4, and Nc4, which reflects their shared common origin with chromosome AK4 of the Ancestral Crucifer Karyotype (n = 8). We argue that multispecies and multi-directional painting will significantly expedite comparative cytogenomics in Brassicaceae and other plant families. |
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