Plasma cell phenotype correlation with cytogenetic and morphological findings in multiple myeloma
| Autoři | |
|---|---|
| Rok publikování | 2011 |
| Druh | Konferenční abstrakty |
| Fakulta / Pracoviště MU | |
| Citace | |
| Popis | Plasma cells (PCs) phenotype could correlate with presence of genetic abnormalities and/or morphological subtypes thus could be related to patient’s prognosis as well. The aim of work was to find relation between antigenic profile, cytogenetic aberrations and morphology in MM patients. Analyses were done in 134 newly diagnosed MM patients. Bone marrow PCs were analysed for expression of CD19, CD20, CD27, CD28, CD56 and CD117 by immunophenotyping. FISH on separated PCs was used for analysis of del(13)(q14), del(17)(p13), IGH disruption, t(4;14)(p16.3;q32), 1q21 gain and hyperdiploidy. PC subtypes evaluation was based on the nucleus/cytoplasm (N/C) ratio. Clonal CD19+ PCs were found in 1 patient with del(13)(q14). CD56+ PCs were found in 79.1% (106/134) and correlated with lower expression of CD20 and CD28. CD56+ PCs were more immature than CD56- PCs. CD20+ PCs were found in 8.2% (11/134) and correlated with higher expression of CD28, CD117 and lower expression of CD56. CD27 was less expressed in group with higher number of CD56+ PCs and CD27+ PCs were more mature. CD28 was expressed in 24.6% (33/134). CD28- PCs were more immature than CD28+ PCs. CD117+ PCs was found in 31.3% (42/134)and significantly correlated with hyperdiploidy, negativity for CD117 was associated with del(13)(q14). Result showed CD117 as the only marker corresponding to chromosomal abnormalities. According to morphology assessment majority of PCs was more mature type. |
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