Analysis of Biomolecules through Surface-Assisted Laser Desorption/Ionization Mass Spectrometry Employing Nanomaterials

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Publikace nespadá pod Filozofickou fakultu, ale pod Středoevropský technologický institut. Oficiální stránka publikace je na webu muni.cz.
Název česky Analýza Biomolekul Hmotnostní spektrometrií s laserovou desorpcí za účasti nanomateriálů
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CHEN Wen-Tsen TOMALOVÁ Iva PREISLER Jan CHANG Huan-Tsung

Rok publikování 2011
Druh Článek v odborném periodiku
Časopis / Zdroj Journal of the Chinese Chemical Society
Fakulta / Pracoviště MU

Středoevropský technologický institut

Citace
Doi http://dx.doi.org/10.1002/jccs.201190120
Obor Analytická chemie, separace
Klíčová slova Nanomaterials; Biomolecules; Surface-assisted laser desorption/ionization mass spectrometry; Mass spectrometry imaging
Popis Nanomaterials, primarily nanoparticles (NPs), can serve as an alternative matrix for the analysis of various biomolecules through SALDI MS. SALDI MS has been developed to overcome poor reproducibility and high background in the low mass region commonly occurring in MALDI MS. Various nanomaterials, unmodified or functionalized with recognition ligands, can have a strong affinity toward certain analytes and thus are applicable for their concentration and enrichment from complex biological matrices. In mass spectrometry imaging (MSI), the use of NPs instead of the conventional matrices can improve the spatial resolution up to the cellular level. In this review, the nature of NPs, the methods of sample preparation and approaches for quantitation of biomolecules through SALDI MS are discussed. Practical applications and limitations of SALDI MS employing NPs for separate samples and MSI are mentioned. With regard to the nature of MSI analysis, the use of nanostructured surfaces for MSI is also reflected in this review.
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