Evolution and function of non-SMC subunits of SMC5/6 complex
| Název česky | Evoluce a funkce non-SMC podjednotek SMC5/6 komlexu |
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| Autoři | |
| Rok publikování | 2013 |
| Druh | Konferenční abstrakty |
| Citace | |
| Popis | Structural Maintenance of Chromosomes (SMC) proteins are highly conserved from bacteria to humans and play fundamental roles in chromosome dynamics, gene regulation and DNA repair. SMC1/3 (cohesin) complex is involved in sister chromatid cohesion, SMC2/4 (condensin) contributes to chromosome condensation and SMC5/6 complex is involved in several DNA repair pathways. SMC5/6 heterodimer associates with four non-SMC elements (Nse). The human Nse3 ortholog MAGEG1, founding member of MAGE (Melanoma Antigen Gene) protein family, interacts with both NSE1 (RING-finger containing protein) and NSE4 subunits. The NSE1-MAGEG1-NSE4 subcomplex bridges the head domains of Smc5-Smc6 heterodimer. In our work we have determined interacting domains of Nse3/MAGEG1 and Nse4/EID proteins. The conservation of interacting surface suggests their tight co-evolution. Functional analysis showed involvement of both protein families in transcription regulation and uncovered one possible function of enigmatic MAGE proteins. |
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