Determination of asymmetric and symmetric dimethylarginines in human plasma using high-performance liquid chromatography with electrochemical detection
Název česky | Stanovení asymetrického a symetrického dimethylargininu v lidské plazmě pomocí HPLC s elektrochemickou detekcí |
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Autoři | |
Rok publikování | 2013 |
Druh | Článek v odborném periodiku |
Časopis / Zdroj | Journal of Separation Science |
Fakulta / Pracoviště MU | |
Citace | |
www | http://onlinelibrary.wiley.com/doi/10.1002/jssc.201300813/abstract |
Doi | http://dx.doi.org/10.1002/jssc.201300813 |
Obor | Biochemie |
Klíčová slova | L Arginine; Asymmetric dimethylarginine; Electrochemical detection; HPLC; Symmetric dimethylarginine |
Přiložené soubory | |
Popis | A new HPLC method was developed and validated for the determination of asymmetric and symmetric dimethylarginines and L arginine in human plasma. After a SPE and evaporation of the eluate, the samples were derivatized with an o-phthaldialdehyde reagent containing 3 mercaptopropionic acid. The derivatives formed were analysed by an isocratic RP HPLC and electrochemical detection at +320 mV. |
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