Determination of asymmetric and symmetric dimethylarginines in human plasma using high-performance liquid chromatography with electrochemical detection

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Authors	KLEPÁRNÍK Martin TOMANDLOVÁ Marie GLATZ Zdeněk TOMANDL Josef
Year of publication	2013
Type	Article in Periodical
Magazine / Source	Journal of Separation Science
MU Faculty or unit	Faculty of Medicine
Citation
Web	http://onlinelibrary.wiley.com/doi/10.1002/jssc.201300813/abstract
Doi	http://dx.doi.org/10.1002/jssc.201300813
Field	Biochemistry
Keywords	L Arginine; Asymmetric dimethylarginine; Electrochemical detection; HPLC; Symmetric dimethylarginine
Attached files	EL_determination.pdf
Description	A new HPLC method was developed and validated for the determination of asymmetric and symmetric dimethylarginines and L arginine in human plasma. After a SPE and evaporation of the eluate, the samples were derivatized with an o-phthaldialdehyde reagent containing 3 mercaptopropionic acid. The derivatives formed were analysed by an isocratic RP HPLC and electrochemical detection at +320 mV.
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