Portable capillary electrophoresis instrument with contactless conductivity detection for on-site analysis of small volumes of biological fluids

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Publikace nespadá pod Filozofickou fakultu, ale pod Středoevropský technologický institut. Oficiální stránka publikace je na webu muni.cz.
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GREGUŠ Michal FORET František KUBÁŇ Petr

Rok publikování 2016
Druh Článek v odborném periodiku
Časopis / Zdroj Journal of Chromatography A
Fakulta / Pracoviště MU

Středoevropský technologický institut

Citace
www http://www.sciencedirect.com/science/article/pii/S0021967315017525
Doi http://dx.doi.org/10.1016/j.chroma.2015.11.088
Obor Analytická chemie, separace
Klíčová slova Portable capillary electrophoresis; Contactless conductivity detection; Low sample volume; Exhaled breath condensate; Methanol intoxication
Popis A novel, easy to use and portable capillary electrophoretic instrument for injection of small volumes of biological fluids equipped with contactless conductivity detection was constructed. The instrument is lightweight(<5 kg), all necessary parts including a tablet computer are accommodated in a plastic briefcase with dimensions 20 cm x 33 cm x 17 cm (w x l x h), allows hydrodynamic injection of small sample volumes and can continuously operate for at least 10 hours. The semi-automated hydrodynamic sample injection is accomplished via a specially designed PMMA interface that is able to repeatedly inject sample aliquots from a sample volume as low as 10 mu l, with repeatability of peak areas below 5%. The developed interface and the instrument were optimized for the injection of biological fluids. Practical utility was demonstrated on the determination of formate in blood serum samples from acute methanol intoxication patients and on the analysis of ionic profile (nitrosative stress markers, including nitrite and nitrate) in the exhaled breath condensate from one single exhalation. (C) 2015 Elsevier B.V. All rights reserved.
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