Portable capillary electrophoresis instrument with contactless conductivity detection for on-site analysis of small volumes of biological fluids

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Authors

GREGUŠ Michal FORET František KUBÁŇ Petr

Year of publication 2016
Type Article in Periodical
Magazine / Source Journal of Chromatography A
MU Faculty or unit

Central European Institute of Technology

Citation
Web http://www.sciencedirect.com/science/article/pii/S0021967315017525
Doi http://dx.doi.org/10.1016/j.chroma.2015.11.088
Field Analytic chemistry
Keywords Portable capillary electrophoresis; Contactless conductivity detection; Low sample volume; Exhaled breath condensate; Methanol intoxication
Description A novel, easy to use and portable capillary electrophoretic instrument for injection of small volumes of biological fluids equipped with contactless conductivity detection was constructed. The instrument is lightweight(<5 kg), all necessary parts including a tablet computer are accommodated in a plastic briefcase with dimensions 20 cm x 33 cm x 17 cm (w x l x h), allows hydrodynamic injection of small sample volumes and can continuously operate for at least 10 hours. The semi-automated hydrodynamic sample injection is accomplished via a specially designed PMMA interface that is able to repeatedly inject sample aliquots from a sample volume as low as 10 mu l, with repeatability of peak areas below 5%. The developed interface and the instrument were optimized for the injection of biological fluids. Practical utility was demonstrated on the determination of formate in blood serum samples from acute methanol intoxication patients and on the analysis of ionic profile (nitrosative stress markers, including nitrite and nitrate) in the exhaled breath condensate from one single exhalation. (C) 2015 Elsevier B.V. All rights reserved.
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