Unusual puzzle – retention properties of nucleobases and nucleotides as elements for retention model of ultrashort oligonucleotides

Varování

Publikace nespadá pod Filozofickou fakultu, ale pod Přírodovědeckou fakultu. Oficiální stránka publikace je na webu muni.cz.

Autoři	TOMICZKOVÁ Petra BITTOVÁ Miroslava HAVLIŠ Jan
Rok publikování	2019
Druh	Konferenční abstrakty
Fakulta / Pracoviště MU	Přírodovědecká fakulta
Citace
Popis	To construct functional model of sequence-dependent separation of ultrashort oligonucleotides in HILIC, we have to consider linear contribution of partial structures (nucleobase, ribose, monophosphate) to overall retention. Within the problem, we consider the nucleobase and phosphate to have the highest impact. To optimally analyse the retention behaviour of ultrashort oligonucleotides in regard to their structure, we have acquired retention factors of individual nucleobases (U, T, A, G a C) and respective nucleotide monophosphates (NMP) under defined conditions, i.e. constant content of organic component (80% ACN), variable pH (1 – 6) and TEAP concentration (20 and 100 mM).