Unusual puzzle – retention properties of nucleobases and nucleotides as elements for retention model of ultrashort oligonucleotides

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Authors	TOMICZKOVÁ Petra BITTOVÁ Miroslava HAVLIŠ Jan
Year of publication	2019
Type	Conference abstract
MU Faculty or unit	Faculty of Science
Citation
Description	To construct functional model of sequence-dependent separation of ultrashort oligonucleotides in HILIC, we have to consider linear contribution of partial structures (nucleobase, ribose, monophosphate) to overall retention. Within the problem, we consider the nucleobase and phosphate to have the highest impact. To optimally analyse the retention behaviour of ultrashort oligonucleotides in regard to their structure, we have acquired retention factors of individual nucleobases (U, T, A, G a C) and respective nucleotide monophosphates (NMP) under defined conditions, i.e. constant content of organic component (80% ACN), variable pH (1 – 6) and TEAP concentration (20 and 100 mM).