Calibration of Chiral Compounds with Self-Internal Standard. Application to Determination of S(-)-propranolol Hydrochloride by Capillary Zone Electrophoresis
| Autoři | |
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| Rok publikování | 2002 |
| Druh | Článek v odborném periodiku |
| Časopis / Zdroj | Chromatographia |
| Fakulta / Pracoviště MU | |
| Citace | |
| Obor | Analytická chemie, separace |
| Klíčová slova | Capillary electrophoresis; Chiral separation; beta-cyclodextrin; Racemic switch |
| Popis | A new method of calibration designed for chiral compounds named: Calibration with Self-internal Standard, is suggested. Here, the second enantiomer serves as the internal standard, the total concentration of both enantiomers is known and kept constant. The method is an alternative to the commonly used calibration with an internal standard being a chemically different compound. The applicability of the method is demonstrated on the calibration of propranolol hydrochloride in capillary zone electrophoresis. Statistical characteristics of the method are compared to calibration without an internal standard and with L-tryptophan as internal standard. |
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