Calibration of Chiral Compounds with Self-Internal Standard. Application to Determination of S(-)-propranolol Hydrochloride by Capillary Zone Electrophoresis

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Authors

PAZOUREK Jiří PERRIN Cathrin MASSART Luc

Year of publication 2002
Type Article in Periodical
Magazine / Source Chromatographia
MU Faculty or unit

Faculty of Informatics

Citation
Field Analytic chemistry
Keywords Capillary electrophoresis; Chiral separation; beta-cyclodextrin; Racemic switch
Description A new method of calibration designed for chiral compounds named: Calibration with Self-internal Standard, is suggested. Here, the second enantiomer serves as the internal standard, the total concentration of both enantiomers is known and kept constant. The method is an alternative to the commonly used calibration with an internal standard being a chemically different compound. The applicability of the method is demonstrated on the calibration of propranolol hydrochloride in capillary zone electrophoresis. Statistical characteristics of the method are compared to calibration without an internal standard and with L-tryptophan as internal standard.
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