Project information
Human genome structure
- Project Identification
- IAA5004306
- Project Period
- 1/2003 - 12/2007
- Investor / Pogramme / Project type
-
Academy of Sciences of the Czech Republic
- Grants of distinctly investigative character focused on the sphere of research pursued at present particularly in the ASCR
- MU Faculty or unit
- Faculty of Informatics
- Keywords
- nuclear architecture; chromosome structure; FISH; high-resolution cytometry;
- Cooperating Organization
-
Institute of Biophysics
- Responsible person doc. RNDr. Stanislav Kozubek, DrSc.
One of the main directions of human genome research in the post-genomic eta is the study of its higher-order structure that became the key to understanding of the most important cellular processes such as cell differentiation or transformation. Using high-resolution cytometry developed in our laboratory, nuclear and territorial topography of genetic elements will be systematically investigated in normal and malignant human cell types. The topography and its changel will be correlated with local acetylation of histones, methylation of DNA and expression of gene clusters. In addition, changes of the structure of chromosome terriories or whole human genome in the cell nucleus during differentiation and cancerogenesis will be determined and an attempt will be made to find the basic psrinciples of the epigenetic regulation of gene expression. The practical goal of the project is the use of experimental results and technology developed in our laboratory in diagnostics ant therapy of cancer.
Results
The practical goal of the project is the use of experimental results and technology developed in our laboratory in diagnostics and therapy of cancer.
Publications
Total number of publications: 32
2004
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Applications of Image Registration in Human Genome Research
Computer Vision and Mathematical Methods in Medical and Biomedical Image Analysis, year: 2004
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Automated acquisition and processing of multidimensional image data in confocal in vivo microscopy
Microscopy research and technique, year: 2004, volume: 64, edition: 2
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Automated confocal microscopy: The way of achieving both quality and quantity in 3D image cytometry
International Symposium on Biomedical Imaging, year: 2004
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Automated confocal microscopy: The way of achieving both quality and quantity in 3D image cytometry
Year: 2004, type:
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Automated image analysis in fluorescence microscopy: From isolated cells to tissues and microarray images
Biophysics of the Genome, year: 2004
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Colocalization of PML bodies and PML/RARalpha microspeckles with up- and down-regulated loci and changes of chromatin structure in APL leukemia cells
Biophysics of the Genome, year: 2004
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Cytological study of DNA methylation and histone methylation patterns in human cell lines
Biophysics of the Genome, year: 2004
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Different nuclear localization of aberrant and normal chromosome 8 homologues in intact or NaBt differentiated HT-29 cells
Biophysics of the Genome, year: 2004
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Dynamics of HP1 transgene loci movement and silencing in living cells
Biophysics of the Genome, year: 2004
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From Jenalumar to Ultrafast Microscopy of Living Cells
Biophysics of the Genome, year: 2004