Project information
Activity of acidophilic sulfur-oxidizing bacteria under aerobic and anaerobic conditions
- Project Identification
- GA525/08/0697
- Project Period
- 1/2008 - 5/2012
- Investor / Pogramme / Project type
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Czech Science Foundation
- Standard Projects
- MU Faculty or unit
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Faculty of Science
- doc. Ing. Martin Mandl, CSc.
- doc. Mgr. Pavel Bouchal, Ph.D.
- doc. RNDr. Oldřich Janiczek, CSc.
- Keywords
- sulfur-oxidizing bacteria, acid mine drainage, sulfide wastes
The project proposal investigates activity of sulfur-oxidizing bacteria to obtain new results on process parameters which results in environmental acidification and toxic metal mobilization in sulfide wastes and abandoned mines. An Acidithiobacillus ferrooxidans mine isolate will be a model bacterium to study elemental sulfur oxidation. To learn more about the cell energetic profile, a method for ATP determination will be applied in relation to oxidation kinetics and assessment of an actual metabolic potential of bacteria to form sulfuric acid. The aerobic bacteria will be adapted to anaerobic sulfur oxidation to study process parameters under different physiological state of bacteria. Proteome analysis of aerobic and anaerobic bacteria will be performed to obtain knowledge on characteristic proteins which are formed during elemental sulfur oxidation and a cell switch to iron oxidation. This will provide a complex view to the metabolic control. The project can contribute to the study of physiology of sulfur-oxidizing bacteria in relation to their role in the environment.
Publications
Total number of publications: 22
2011
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Anaerobic oxidation of elemental sulfur in the chemolithoautotrophic bacterium Acidithiobacillus ferrooxidans
Year: 2011, type: Conference abstract
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Application of a bioluminescent ATP assay for monitoring growth of acidophilic bacteria
Year: 2011, type: Conference abstract
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Biodegradation of iron sulfides by acidophilic bacteria and effects of released iron on the environment
Year: 2011, type: Conference abstract
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Bioluminescent assay as a tool for monitoring metabolic activity of acidophilic bacteria
Year: 2011, type: Conference abstract
2010
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Metabolic changes in sulfur- and iron-oxidizing Acidithiobacillus ferrooxidans
Year: 2010, type: Conference abstract
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Mobilization and phytotoxicity of iron under conditions of bacterial oxidation of iron sulfides
Year: 2010, type: Conference abstract
2009
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ATP measurements in iron-oxidizing Acidithiobacillus ferrooxidans
Advanced Materials Research, year: 2009, volume: 71-73, edition: 6
2008
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Acidofilní sirné a železité bakterie v ekologii sulfidů.
Year: 2008, type: Conference abstract
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Growth kinetics of sulfur-oxidizing bacteria and kinetic models
Year: 2008, type: Conference abstract
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Impacts of growth kinetics of sulfur-oxidizing Acidithiobacillus ferrooxidans on kinetic models
Year: 2008, type: Conference abstract