Project information
Analysis of gastrointestinal microbiome in patients with primary immune deficiencies
- Project Identification
- MUNI/M/1322/2015
- Project Period
- 3/2016 - 12/2018
- Investor / Pogramme / Project type
-
Masaryk University
- Grant Agency of Masaryk University
- INTERDISCIPLINARY - Interdisciplinary research projects
- MU Faculty or unit
- Faculty of Medicine
- Other MU Faculty/Unit
- Faculty of Informatics
- Other MU Faculty/Unit
- Central European Institute of Technology
In the past decade, gut microbiota has been shown to be associated with a number of human diseases including metabolic syndrome, obesity-related diseases, inflammatory bowel disease, and colorectal cancer. In this project, we plan to study human microbiome in primary antibody immune deficiencies, common variable immunodeficiency (CVID) and selective IgA deficiency (IgAD). In both diseases, IgA production is seriously affected. Since IgA is the dominant mucosal immunoglobulin and plays a central role in maintaining intestinal homeostasis, it is expected that i) the gut microbiome reflects changes in host mucosal immune system. In addition, since there is a significant portion of patients without clinical symptoms or with very mild symptoms, it is likely that ii) the microbiome composition is of importance in these inapparent disease cases. To determine microbiome composition, 16S rRNA gene sequences from the gut microbiome will be examined in all DNA samples (n =50) isolated from patients and healthy controls. In addition, analysis of gut metagenome by whole-metagenome shotgun sequencing and subsequent analysis of gut metabolome will be performed in 6-10 samples previously characterized by the analysis of 16S rRNA gene sequences. This will allow characterization of microbial communities present in the samples and determination of alpha and beta diversity of the samples. In addition, functional characterization of human microbiome will be performed together with determination of the host’s metabolome in a number of selected samples.
Publications
Total number of publications: 11
2021
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Patients With Common Variable Immunodeficiency (CVID) Show Higher Gut Bacterial Diversity and Levels of Low-Abundance Genes Than the Healthy Housemates
Frontiers in Immunology, year: 2021, volume: 12, edition: May 2021, DOI
2020
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Administration of the Probiotic Escherichia coli Strain A0 34/86 Resulted in a Stable Colonization of the Human Intestine During the First Year of Life
Probiotics and Antimicrobial Proteins, year: 2020, volume: 12, edition: 2, DOI
2019
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Bacterial and fungal gut microbiota analysis in common variable immunodeficiency (CVID) disorders: paired case-control study
Year: 2019, type: Conference abstract
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Bacterial but Not Fungal Gut Microbiota Alterations Are Associated With Common Variable Immunodeficiency (CVID) Phenotype
Frontiers in Immunology, year: 2019, volume: 10, edition: 1914, DOI
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Gut microbiome analysis in common variable immunodeficiency (CVID) patients and paired healthy controls sharing the same household
Year: 2019, type: Conference abstract
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The Impact of DNA Extraction Methods on Stool Bacterial and Fungal Microbiota Community Recovery
Frontiers in Microbiology, year: 2019, volume: 10, edition: 821, DOI
2018
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Analýza mykobiomu aneb chybami se člověk učí.
Year: 2018, type: Conference abstract
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Artificial fungal community (fMOCK) as a control of sequencing process in mycobiome research.
Year: 2018, type: Conference abstract
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Gut microbiome analysis in common variable immunodeficiency (CVID) patients.
Year: 2018, type: Conference abstract
2017
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Comparison of five bacterial DNA extraction methods from germ-free mice fecal samples
Year: 2017, type: Conference abstract