Application of On-line Capillary Electrophoresis Coupled to Mass Spectrometry for Identification of Cytochrome P450 2C9 Isoform Metabolism Products
| Authors | |
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| Year of publication | 2012 |
| Type | Conference abstract |
| MU Faculty or unit | |
| Citation | |
| Description | Drug biotransformation mediated by cytochrome P450 enzymes (CYP) is a pivotal factor in the early developmental stages of new drugs. Metabolism appraisal of every hit thus constitutes the inseparable part of ADME/tox screenings. Capillary electrophoresis (CE) represents a promising technique in the field of enzyme assays due to miniscule sample consumption and high-throughput by automation. Moreover, CE coupled to mass spectrometry (MS) combines the advantages of both techniques so that CYP metabolism products can be identified on the bases of migration time, molecular weight and fragmentation. A new CE-MS method enabling identification of reaction products provided by clinically important CYP2C9 isoform is thus introduced. |
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