Analysis of chromosomal abnormalities in phenotypically normal plasma cells detected by I-FISH in monoclonal gammopathy of undetermined significance
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| Year of publication | 2012 |
| Type | Conference abstract |
| MU Faculty or unit | |
| Citation | |
| Description | Monoclonal gammopathy of undetermined significance (MGUS) is a premalignant stage of multiple myeloma. MGUS consists of phenotypically normal (nPCs; CD138+19+56-) and abnormal malign plasma cells (aPCs; CD138+19-56+/-). Combination of fluorescence activated cell sorting (FACS) and interphase fluorescence in situ hybridization (I-FISH) allows monitoring of specific chromosomal abnormalities in separate PCs populations. We hypothesized that there should not be any chromosomal abnormalities in nPCs. By I-FISH we examined following chromosomal alterations in nPCs and aPCs: del(13)(q14), del(17)(p13), IGH rearrangement, 1q21 gain and hyperdiploidy (+5, +9 and +15). In this study, we chose MGUS patients from whom it was possible to separate nPCs and aPCs and who had IGH rearrangement in aPCs (n=15). In the nPCs we found only IGH disruption. Total of 27% (4/15) and 73% (11/15) MGUS patients have more than 20% and 10% aberrant PCs bearing IGH rearrangement not only in aPCs, but also in nPCs, respectively. Other chromosomal abnormalities were detected only in aPCs: del(13)(q14) was found in 20% (3/15), 1q21 gain in 7% (1/15) and hyperdiploidy in 13% (2/15). In conclusion, we found IGH rearrangement in phenotypically nPCs defined by CD138+CD19+CD56- and thus we assume that the separation according to this phenotype is not sufficinent to separate genetically nPCs. |
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