The DNA and RNA sugar–phosphate backbone emerges as the key player. An overview of quantum-chemical, structural biology and simulation studies

Investor logo
Investor logo

Warning

This publication doesn't include Faculty of Arts. It includes Central European Institute of Technology. Official publication website can be found on muni.cz.
Authors

ŠPONER Jiří MLÁDEK Arnošt ŠPONEROVÁ Judit SVOZIL Daniel ZGARBOVÁ Marie BANÁŠ Pavel JUREČKA Petr OTYEPKA Michal

Year of publication 2012
Type Article in Periodical
Magazine / Source PHYSICAL CHEMISTRY CHEMICAL PHYSICS
MU Faculty or unit

Central European Institute of Technology

Citation
Web http://www.ncbi.nlm.nih.gov/pubmed/23072945
Doi http://dx.doi.org/10.1039/c2cp41987d
Field Biophysics
Keywords HEPATITIS-DELTA-VIRUS; MOLECULAR-DYNAMICS SIMULATIONS; NUCLEIC-ACID BACKBONE; DENSITY-FUNCTIONAL CALCULATIONS; INTRAMOLECULAR HYDROGEN-BONDS; SINGLE-STRAND BREAKS; INTRINSIC CONFORMATIONAL PROPERTIES; MAIN-GROUP THERMOCHEMISTRY; SELF-CLEAVING RIBOZYMES
Attached files
Description Knowledge of geometrical and physico-chemical properties of the sugar–phosphate backbone substantially contributes to the comprehension of the structural dynamics, function and evolution of nucleic acids. We provide a side by side overview of structural biology/bioinformatics, quantum chemical and molecular mechanical/simulation studies of the nucleic acids backbone. We highlight main features, advantages and limitations of these techniques, with a special emphasis given to their synergy. The present status of the research is then illustrated by selected examples which include classification of DNA and RNA backbone families, benchmark structure–energy quantum chemical calculations, parameterization of the dihedral space of simulation force fields, incorporation of arsenate into DNA, sugar–phosphate backbone self-cleavage in small RNA enzymes, and intricate geometries of the backbone in recurrent RNA building blocks. Although not apparent from the current literature showing limited overlaps between the QM, simulation and bioinformatics studies of the nucleic acids backbone, there in fact should be a major cooperative interaction between these three approaches in studies of the sugar–phosphate backbone.
Related projects:

You are running an old browser version. We recommend updating your browser to its latest version.