Vztah rodičovských genomů u mezidruhových hybridů Trifolium pratense x Trifolium medium

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Title in English Intergenomic relationships in interspecific hybrids of Trifulium pratense x Trifolium medium
Authors

ŘEPKOVÁ Jana SIMANDLOVÁ Jana JAKEŠOVÁ Hana NEDĚLNÍK Jan SOLDÁNOVÁ Martina HAJŠLOVÁ Jana SCHULZOVÁ Věra

Year of publication 2012
Type Article in Periodical
Magazine / Source Úroda, Vědecká příloha
MU Faculty or unit

Faculty of Science

Citation
Field Genetics and molecular biology
Keywords Flow cytometry Genomic in situ hybridisation Trifolium medium Trifolium pratense
Description Breeding material derived from hybrids Trifolium pratense x T. medium is a promising source of new genetic variability for cultivated red clover (7. pratense). These hybrids were evaluated on the cytological level via flow cytometry and genomic in situ hybridization. Plants of 99 sources in two breeding nurseries had comparable chromosome numbers in five groups: 2n : 28 (76 and 66% of plants), bellow of 28 chromosomes (8.5 and6.4oh of plants),2n:29,30 (15 and 26% of plants),2n: 31, 40 (0.4 and 1.2% of plants) and 2n: 4I to 46 (0.1 and 0.3% of plants). The identification of introduced chromosomes or translocated parts was unsuccessful. Hybrids contained lover level of phytoestrogens than T. pratense but increased amount of formononetin with comparison of T. medium was evaluated. Interspecific variability will be identified by genomic procedures based on de novo sequencing of the both parental genome's.
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