Metabolomics: nucleotide and coenzyme determination in cells by capillary electrophoresis

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Title in English	Metabolomics: nucleotide and coenzyme determination in cells by capillary electrophoresi
Authors	MUSILOVÁ Jindra GLATZ Zdeněk
Year of publication	2012
Type	Article in Proceedings
Conference	9th International Interdisciplinary Meeting on Bioanalysis, CECE 2012
MU Faculty or unit	Faculty of Science
Citation
Field	Biochemistry
Keywords	capillary electrophoresis; metabolomics
Description	Capillary electrophoresis (CE) is popular and suitable method for the metabolome analysis particularly due to high-efficient separations of a diverse range of chemical compounds, short analysis times and low sample volume requirements. New separations conditions for selective and rapid determination of 17 energetically important metabolites by CE were optimized. Applicability of this new method to real samples was demonstrated on bacterium P. denitrificans and stem cells extracts.
Related projects:	Capillary electrophoresis as a member of the metabolomic analytical toolbox Centrum pokročilých bioanalytických technologií