"Click & Seed" Approach to the Biomimetic Modification of Material Surfaces
| Authors | |
|---|---|
| Year of publication | 2012 |
| Type | Article in Periodical |
| Magazine / Source | Macromolecular Bioscience |
| MU Faculty or unit | |
| Citation | |
| Doi | http://dx.doi.org/10.1002/mabi.201200095 |
| Field | Genetics and molecular biology |
| Keywords | biomimetic modifications; click chemistry; peptide radiolabeling; polydopamine; poly(ethylene oxide) |
| Description | A simple, versatile, protein-repulsive, substrate-independent biomimetic surface modification is presented that is based on the creation of a PEO brush on a polydopamine anchoring layer and its capacity for selective follow-up modifications with various ligands using a coppercatalyzed alkyne-azide cycloaddition reaction. The desired surface concentration of peptide biomimetic ligands can be controlled by adjusting the peptide concentration in the reaction mixture, then measuring the activity of 125I-radiolabeled peptides that are immobilized on the substrates. The performance of the prepared substrates is tested in cell cultures with MEF cells and a human ECC line. |
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