ABP1 and ROP6 GTPase Signaling Regulate Clathrin-Mediated Endocytosis in Arabidopsis Roots
| Authors | |
|---|---|
| Year of publication | 2012 |
| Type | Article in Periodical |
| Magazine / Source | Current Biology |
| MU Faculty or unit | |
| Citation | |
| Web | http://www.cell.com/current-biology/retrieve/pii/S0960982212005738 |
| Doi | http://dx.doi.org/10.1016/j.cub.2012.05.020 |
| Field | Genetics and molecular biology |
| Keywords | AUXIN-EFFLUX; PLANT-CELLS; PLASMA-MEMBRANE; TRANSPORT; POLARITY; EXPRESSION; DYNAMICS; CARRIER; PIN2 |
| Description | The dynamic spatial and temporal distribution of the crucial plant signaling molecule auxin is achieved by feedback coordination of auxin signaling and intercellular auxin transport pathways [1, 2]. Developmental roles of auxin have been attributed predominantly to its effect on transcription; however, an alternative pathway involving AUXIN BINDING PROTEIN1 (ABP1) has been proposed to regulate clathrin-mediated endocytosis in roots and Rho-like GTPase (ROP)-dependent pavement cell interdigitation in leaves [3, 4]. In this study, we show that ROP6 and its downstream effector RIC1 regulate clathrin association with the plasma membrane for clathrin-mediated endocytosis, as well as for its feedback regulation by auxin. Genetic analysis revealed that ROP6/RIC1 acts downstream of ABP1 to regulate endocytosis. This signaling circuit is also involved in the feedback regulation of PIN-FORMED 1 (PIN1) and PIN2 auxin transporters activity (via its constitutive endocytosis) and corresponding auxin transport-mediated processes, including root gravitropism and leave vascular tissue patterning. Our findings suggest that the signaling module auxin ABP1 ROP6/RIC1 clathrin PIN1/PIN2 is a shared component of the feedback regulation of auxin transport during both root and aerial development. |
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