Možnosti studia enzymových reakcí pomocí kapilární elektroforézy v on-line uspořádání

• Title in English: Study of Enzymatic Reaction by On-line Capillary Electrophoresis.
• Authors: ŘEMÍNEK Roman; ZEISBERGEROVÁ Marta; GLATZ Zdeněk
• Year of publication: 2013
• Type: Article in Periodical
• Magazine / Source: Chemické listy
• MU Faculty or unit: Faculty of Science
• Field: Biochemistry
• Keywords: metabolomics; metabolome; metabolism; NMR; MS; GS; HPLC; CE
• Description: This review provides overview of techniques used for merging of consecutively injected plugs of reactants within in-capillary enzymatic assays. The article is divided as follows: after an introduction, general features of on-line capillary electrophoresis and principles and applications of electrophoretically mediated microanalysis, at-inlet reactions and transverse diffusion of laminar flow profiles techniques are consecutively discussed and illustrated. Future directions are outlined in conclusion.

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